Electron Cryo-Microscopy and 3D Image Reconstruction of Viruses and Virus Complexes

A1. Nature of Projects
A2. Summary of Accomplishments
A3. Project Organization
A4. Future Directions

B1. Preliminary Considerations/Questions Regarding the Nature of the Specimen
B2. EM Grid Preparation
B3. Negative Staining
B4. Specimen Vitrification

C. MICROSCOPY

C1. Preliminary Considerations
C2. Microscope Operation, Alignment and Calibration
C3. Recording Devices Handling
C4. Photography
C5. Data Archival

D. STRUCTURE DETERMINATION (Macromedia Flash Required)

D1. Preliminary Considerations
D2. Computing Environment
D3. 3D Reconstruction Protocol (of Icosahedral Viruses)
D4. Miscellaneous Issues

E. DATA VISUALIZATION AND INTERPRETATION

E1. Visualization Tools
E2. Data Format Issues and Conversion Programs
E3. Difference Maps
E4. Fitting Models to 3D Reconstructions
E5. Correlating 3D Reconstruction Results with Other Data
E6. Predictions Based on Pseudo-Atomic Models
E7. Rendering X-ray Structures at Low Resolution

F. DISSEMINATION OF RESULTS

F1. Primary Publications
F2. Seminar Presentations
F3. Conferences
F4. News Media

G. DATA ARCHIVAL

Abbreviations:

3D	three-dimensional
cryoEM	electron cryo-microscopy
keV	kilo electron volts
FEG	field emission gun
TEM	transmission electron microscope/microscopy